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Monday, 5 November 2012

AIM 2 - Biological Survey

Bacteria
  • -          Autotrophic & heterotrophic marine bacteria
  • -          < 2 um, Microscopic, unicellular
  • -          Removed from water by 0.45 μm/0.2 μm
Plankton
-          Phytoplankton
o   Abundant, widely distributed
o   Microscopic floating plant, < 100 m from surface
o   2 – 200 μm
o   Plankton net tow 60 μm (count & biomass determination)
-          Collecting phytoplankton
o   Standard conical net = fine mesh & 1m ø mouth
o   Towed behind ship at set distance
o   Number of organisms present / species diversity = organism count & filtered volume of water
o   Primary productivity determination = chlorophyll-a determination

-          Zooplankton
o   Small herbivorous / carnivorous animals
o   Feed on phytoplankton / zooplankton
o   20 – 5000 μm
o   Bongo net of 333 μm / 500 μm (estimate count, sp. diversity, biomass determination)
o   Limited to upper sunlit zone, but deeper

-          Benthos
o   Trawling technique: beam trawl (burrows)
o   Infaunal (sometimes sedimentary) & epifaunal benthos, size distribution, sampling
o   All depth sediment has epi- & infaunal of different density
o   Size division:
§  Megafaunal >20cm
§  Macrofaunal 20cm – 0.5mm
§  Meiofaunal 0.5mm – 50μm
§  Microfaunal 50μm – 0.5μm



Diversity & Stability
  • -          Community diversity = species number @ a time (mathematically documented)
  • -          High density à High species; few individuals/species
  • -          Diversity indices (quantifies community diversity all environment)

o   Simpson index


     

o   Shannon function 



N = total individuals of all species     
ni = number of individuals in ith species

*Common logarithm bases are 1, 10, & e

  • -          Community stability depends on time & less defined. If diversity, sp. composition changed little, then community is stable.
  • -          Diversity Index esp. used in transects to examine impact of pollutants (land pollution on coastal water & oily water from oil field)
Coral reef

  • -          Occupies 1% of oceans
  • -          Shallow warm subtropical & tropical water, >3000 species
  • -          From coral skeleton, <20m
  • -          Zooxanthellae, symbiotics photosynthetic algae (dinoflagellates) live in endoderm cells of coral
Great Barrier Reef

  • -          Stretches 2600km, area 344,400km2, > 2900 individual reefs
  • -          Off Queensland coast
Nekton

  • -          Highest-trophic-level organisms in estuaries/oceans sustains locomotion for prey
  • -          Fish, crustaceans, cephalopods, seabirds, marine reptiles & mammals

Sunday, 4 November 2012

AIM 1 - Sampling


Sampling Stations
  • -          Sites in a large study area
  • -          Meaningful sample results collected according to objective of study/research/project
  • -          Sub-sampling stations at various depth for water hydrological properties

Objective
  • -          To assess impact of activity
  • -          Collect portion of material and transported & handled in lab accurately

Examples
  • -          Kuala Terengganu Estuary for environmental study
  • -          Straits of Malacca for oceanographic study
  • -          Oil field in South China Sea for pollution study

Sampling Techniques
  • -          Need research vessel / boat
  • -          Mostly determined in situ

Hydrolab
  • -          To find temperature, salinity, conductivity, D.O., p.H., depth, turbidity & nutrient

GPS (Global Positioning System)
  • -          To determine the location of sampling station using satellite positioning

Fluorometer
  • -          Dominant phytoplankton has chlorophyll & chlorophyll-a, hence as indicator for phytoplankton & productivity biomass
  • -          Rhodamine (dye) & hydrocarbon

Current Meter
  • -          Current speed & direction (ms-1 & 0-360°)

Echo Sounder
  • -          Depth measurement with sound impulse
  • -          Seawater 1500 ms-1 freshwater 1435ms-1
  • -          Sound velocity in ocean varies with pressure, depth, temperature, salinity

Water Samplers
  • -          Niskin & Van Dorn Sampler
  • -          Collect water sample for chemical analysis

Sediment Samplers
  • -          Ekman grab, Smith McIntyre grab, Phleger corer
  • -          Collect sediment sample for chemical & biological parameter analysis in sediment

Benthic Organisms Sampler
  • -          For sampling benthic organisms, biomass, distribution & diversity studies

Plankton nets
  • -          Sampling planktons
  • -          Bongo net, Norpac net, Kitahara net


IMS 7 - Water & Seawater

Properties of water
-          Chemical recombination restricted by surrounding hydrogen molecules
-          Ice density 0.9170 g/cm3 is ~8% lesser than liquid water
-          Salinity lowers freezing point, salt interferes ordered crystal rearrangement
-          35‰ salinity with freezing point -1.91°C
-          Seawater contains dissolved substance
Salinity
-          Total solid material dissolved, including dissolved gases, excluding suspended fine particles
Seawater constituents
-          70 elements, 6 major elements make 99%: Na, CI, SO4, Mg, Ca, K
Salt Ion
Ions in Seawater (‰)
Ions by Weight (%)
Cumulative (%)
Chloride (Cl-)
18.980
55.04
55.04
Sodium (Na+)
10.556
30.61
85.65
Sulfate (SO42-)
2.649
7.68
93.33
Magnesium (Mg2+)
1.272
3.69
97.02
Calcium (Ca2+)
0.400
1.16
98.18
Potassium (K+)
0.380
1.10
99.28
Bicarbonate (HCO3-)
0.140
0.41
99.69
Bromide (Br-)
0.065
0.19
99.88
Boric Acid (H3BO3)
0.026
0.07
99.95
Strontium (Sr2+)
0.013
0.04
99.99
Fluoride (F-)
0.001
0.00
99.99
Total
34.482
99.99
99.99

Trace Elements
  • -          0.001‰; 1ppm
  • -          manganese (Mn), lead (Pb), gold (Au), iron (Fe), iodine (I)

Seawater composition
  • -          Dissolved gases, Nutrient (N, Si, P), Organic compounds (fat, protein, carbohydrate)
  • -          NO3- and PO43- utilized by photosynthesizers
  • -          Biological uptake & release, non-conservative (highly varied [con])

Non-conservative
  • -          Seawater dissolved substances tied to biological/seasonal/geological cycles (short Residence Times)

Residence Times
  • -          Avr. time a substance remains in specified region of space

Salinity variation
  • -          Avr. 33 – 38ppt
  • -          Baltic Sea 10ppt
  • -          Red Sea & Persian Gulf 42ppt
  • -          Dead Sea 330ppt (hypersaline inland lake)
  • -          Great Salt Lake, Utah 280ppt (hypersaline inland lake)

Salinity Determination
  • -          Evaporate weight amount of seawater & weigh salt
  • -          Ocean well-mixed; Exact same proportion of major dissolved constituents everywhere
  • -          550.4‰ chlorinity; easy to measure
  • -          Salinity (‰) = 1.80655 x Chlorinity (‰) or conductivity

Conductivity
  • -          Measures strength of nutrient solution

Processes Decreasing Salinity
-          Precipitation
-          Runoff
-          Melting icebergs
-          Melting sea ice

Salinity regulator
  • -          River bring dissolved salts 2.5 – 4.5 x 1015 g/yr
  • -          Atmospheric volcanic gas & hydrothermal circulation release cations (Ca2+ , K+) and anions (SO42-, Cl-)


Chemical equilibrium factors
  • -          Chemical precipitation & evaporite minerals (CaCO3, NaCl, CaSO4)

-          Evaporation & supersaturation
  • -          Marine aerosols

-          Salt coatings nearshore
  • -          Ion Adsorption by clay minerals (authigenic mineral formation)

-          Al, Fe, Mg
  • -          Biological precipitation

-          Hard part secretion (Ca2+, Sr2+), Eg: shells
  • -          Drawn into mantle & subduction zone

Gas
Dry Air (%)
Surface Ocean (%)
Nitrogen (N2)
78.03
47.5
Oxygen (O2)
20.99
36.0
Carbon Dioxide (CO2)
0.03
15.1
Hydrogen (H2)
Trace
Trace
Argon (Ar)
Trace
Trace
Neon (Ne)
Trace
Trace
Helium (He)
0.95
1.4

CO2 + H2O + Light » CH2O + O2  (Photosynthesis)
CH2O + O2 » CO2 + H2O + Energy (Respiration)
-          Gas diffusion across air-sea interface & planktonic photosynthesis (high 02)
-          Organic matter accumulation, feeding organism respiration, bacterial decomposition (Low 02)
-          Low BOD, cold gas-saturated water, hydrothermal vent (Increasing 02)
pH = - log10 [H+]

Seawater as Buffer (pH 8)
-          CO2 + H2O à H2CO3 (Mostly)
-          H2CO3 à H+ + HCO3- (Bicarbonate – further dissociation)
-          HCO3- à H+ + CO32- (Carbonate – further dissociation)
-          CO32- + Ca2+ --> CaCO3